Figure 4.

Quercetin reduction of amylin (8–37) cytotoxicity in pancreatic rat insulinoma Rat insulinoma pancreatic (RIN-m5F) β-cells. Cells were treated for 48 h with amylin (75 µM) in the presence or absence of benzbromarone (BBM, 10 µM), quercetin (QR, 10 µM), folic acid (FA, 10 µM), or Congo red (CR, 100 µM), and cell viability was measured using a 2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide: XTT assay. The results obtained were normalized to control cells (dimethyl sulfoxide: DMSO treated) and expressed as mean values ± SD (n = 7 independent experiments). No statistical differences were obtained between the conditions where only the compounds were added and the control. Statistical analysis was carried out using an unpaired t test. * p < 0.05; ** p < 0.01; **** p < 0.0001.