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Expression stability of four reference gene in brown adipose tissue (BAT) evaluated by BestKeeper (n = 12).
| Gene | Geometric Mean (Ct) | Arithmetic Mean (Ct) | Minimum (Ct) | Maximum (Ct) | Standard Deviation (±Ct) | Coefficient of Variance (% Ct) | Correlation Coefficients (r) |
|---|---|---|---|---|---|---|---|
| 36B4 | 23.78 | 23.80 | 20.89 | 25.26 | 0.78 | 3.26 | 0.960 |
| β-actin | 21.42 | 21.46 | 18.48 | 23.94 | 0.94 | 4.38 | 0.965 |
| 18S | 15.93 | 15.96 | 13.74 | 17.99 | 0.75 | 4.68 | 0.933 |
| GAPDH | 22.03 | 22.08 | 18.45 | 24.90 | 1.12 | 5.06 | 0.953 |
