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. 2016 Jun 20;17(6):970. doi: 10.3390/ijms17060970

Figure 4.

Figure 4

Loss of DCX and calretinin expressing cells, and proliferating cells in subgranular zone (SGZ) at 24 h after irradiation. There is a dramatic loss of DCX positive cells ((A–D), DCX, green; DAPI, blue) and calretinin positive cells ((E–J); calretinin cells, arrow, green; NeuN, red; DAPI, blue; arrowhead points to the band of dense calretinin immunoreactive nerve fibers at the inner molecular layer) at 24 h after irradiation. An apoptotic cell in the SGZ demonstrates bromodeoxyuridine (BrdU) incorporation (K,L), BrdU, green; DAPI, blue; arrowhead) whereas two other apoptotic cells show no BrdU immunoreactivity (arrows); BrdU-retained cells in the SGZ almost disappear completely at 24 h after irradiation ((M–P), BrdU, arrows, green; DAPI, blue; mice irradiated immediately after BrdU given every 2 h for 4 doses).