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. 2016 Jun 20;17(6):970. doi: 10.3390/ijms17060970

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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There is upregulation of p53 and p21 in dentate gyrus after irradiation. There is no evidence for p53 immunoreactivity in dentate gyrus of non-irradiated mice (A); after irradiation, p53 nuclear immunoreactivity is seen in dentate gyrus at 8 h ((B), p53, red; DCX, green; DAPI, blue), and some p53 immunoreactive cells are DCX positive ((B), merged, arrow, inset); cells immunoreactive for p53 do not express nestin or GFAP ((C), control; (D), 5 Gy; p53, green; nestin, red; GFAP, white; DAPI, blue; arrows); there is no p21 immunoreactivity observed in non-irradiated dentate gyrus; increase in p21 nuclear immunoreactivity is seen at 8 h after irradiation ((E), 0 Gy; (F), 5 Gy; p21, red; DCX, green; DAPI, blue; (G); 0 Gy; (H), 5 Gy, p21, green; nestin, red; GFAP, white; DAPI, blue). Some cells immunoreactive for p21 express DCX ((F), merged, arrow, inset), and some express both nestin and GFAP ((H), merged, arrow, inset).

There is upregulation of p53 and p21 in dentate gyrus after irradiation. There is no evidence for p53 immunoreactivity in dentate gyrus of non-irradiated mice (A); after irradiation, p53 nuclear immunoreactivity is seen in dentate gyrus at 8 h ((B), p53, red; DCX, green; DAPI, blue), and some p53 immunoreactive cells are DCX positive ((B), merged, arrow, inset); cells immunoreactive for p53 do not express nestin or GFAP ((C), control; (D), 5 Gy; p53, green; nestin, red; GFAP, white; DAPI, blue; arrows); there is no p21 immunoreactivity observed in non-irradiated dentate gyrus; increase in p21 nuclear immunoreactivity is seen at 8 h after irradiation ((E), 0 Gy; (F), 5 Gy; p21, red; DCX, green; DAPI, blue; (G); 0 Gy; (H), 5 Gy, p21, green; nestin, red; GFAP, white; DAPI, blue). Some cells immunoreactive for p21 express DCX ((F), merged, arrow, inset), and some express both nestin and GFAP ((H), merged, arrow, inset).