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. 2016 Jun 20;17(6):973. doi: 10.3390/ijms17060973

Figure 1.

Figure 1

(a) The cell proliferation of normal breast epithelial cells (MCF10A) in presence of gossypol and ApoG2 was detected using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. MCF10A cell were incubated with increasing concentration of gossypol/ApoG2, as indicated, for 72 h. Similarly, the cell proliferation of pancreatic cancer cell line, BxPC3; breast cancer cell line, MDA-MB-231; and prostate cancer cell line, PC3; in presence of increasing concentrations of (b) gossypol and (c) apogossypolone, as indicated, was detected using MTT assay. The cells were incubated for 72 h. MTT assay was performed as described in the Materials and Methods. All results are present as percentage of control (±SEM) of three independent experiments.