Table 1.
Effect of reactive oxygen species (ROS) scavengers on gossypol and ApoG2-induced apoptosis in cancer cell lines. Cancer cell lines were incubated with different ROS scavengers, and treated with gossypol/ApoG2, as described in the Methods. Cell Death Detection ELISA Kit was used to assess the induction of apoptosis, as described above. Gossypol/ApoG2, 5 μM; TU, 700 μM thiourea; SOD, 100 μg/mL superoxide dismutase; Cat, 100 μg/mL catalase. p < 0.05 compared to treated control.
| Cell Lines | Treatment | Apoptosis (Folds) # | Effect of Scavengers (% Inhibition) |
|---|---|---|---|
| MDA-MB-231 | Untreated | 1.0 | |
| Gossypol | 2.7 | – | |
| + TU | 1.65 | 38.89 | |
| + SOD | 1.62 | 40 | |
| + Cat | 1.58 | 41.48 | |
| ApoG2 | 3.4 | 54.41 | |
| + TU | 1.55 | 55.29 | |
| + SOD | 1.52 | 56.47 | |
| + Cat | 1.48 | – | |
| BxPC-3 | Untreated | 1.0 | |
| Gossypol | 2.1 | – | |
| + TU | 1.14 | 45.71 | |
| + SOD | 1.66 | 20.95 | |
| + Cat | 1.49 | 29.05 | |
| ApoG2 | 2.6 | – | |
| + TU | 1.35 | 48.08 | |
| + SOD | 1.63 | 37.31 | |
| + Cat | 1.58 | 39.23 | |
| PC3 | Untreated | 1.0 | |
| Gossypol | 2.3 | – | |
| + TU | 1.13 | 50.87 | |
| + SOD | 1.68 | 26.96 | |
| + Cat | 1.54 | 33.04 | |
| ApoG2 | 2.9 | – | |
| + TU | 1.38 | 52.41 | |
| + SOD | 1.78 | 38.62 | |
| + Cat | 1.53 | 47.24 |
# Fold-apoptosis was calculated relative to untreated control by direct comparison of O.D. values at 405 nm (O.D. value of treatment group/O.D. value of untreated control). Inhibitory effect of scavengers (% Inhibition) was determined by the formula—[(a − b)/a] × 100, where a = fold apoptosis by Gossypol/ApoG2 and b = fold apoptosis in presence of scavenger (TU/SOD/Cat).