Table 1.
Summary of average hydrodynamic size and ζ-potential of three Ag NPs in water and full cell culture medium. Ag NPs suspended in medium were kept in the incubator (37 °C, 95% air/5% CO2) and the pH value of medium kept around 7.4 during the whole incubation process. All data are represented as the mean ± SD (n = 3).
| Sample | Time (h) | Ag-B | Ag-CIT | Ag-PVP | |||
|---|---|---|---|---|---|---|---|
| Conditions | DLS (nm) | ζ-potential (mV) | DLS (nm) | ζ-potential (mV) | DLS (nm) | ζ-potential (mV) | |
| Ultrapure water (Milli Q) | 0 | 58 ± 0.5 | −12.5 ± 0.6 | 58 ± 0.5 | −5.2 ± 0.4 | 79 ± 0.5 | −16.2 ± 0.4 |
| 24 | 58 ± 2.2 | −13.2 ± 0.3 | 57 ± 1.0 | −15 ± 3.2 | 81 ± 0.4 | −14 ± 1.1 | |
| DMEM, 10% FBS | 0 | 50 ± 0.1 | −6.2 ± 1.8 | 51 ± 0.9 | −7.7 ± 1.9 | 70 ± 0.6 | −3.4 ± 1.3 |
| 24 | 62 ± 2.0 | −7.7 ± 1.4 | 54 ± 0.3 | −9.6 ± 1.5 | 63 ± 0.1 | −8.4 ± 0.7 | |
| 48 | 137 ± 5.7 | −8.8 ± 0.4 | 138 ± 20 | −10.4 ± 1.0 | 93 ± 2.1 | −9.2 ± 0.6 | |