Table 4.
Revertant mutants of TA98 and TA100 S. typhimurium after treatment with various doses of purified antifungal compound SH2 of S. hydrogenans DH16.
| Treatment | Dose (μg/plate) | No. of colonies |
|
|---|---|---|---|
| TA98a | TA100a | ||
| Spontaneousb | 24.33 ± 2.0a | 187.33 ± 11.6a | |
| Positive Controlc | 962.33 ± 4.5b | ||
| (NPD) | 20 | ||
| (Sodium azide) | 2.5 | 1103.67 ± 18.2b | |
| Negative controld | 25.0 ± 1.5a | 185.5 ± 0.5a | |
| (DMSO) | |||
| Purified | 50 | 26.0 ± 0.5a | 185.0 ± 1.5a |
| compound SH2e | 100 | 25.33 ± 1.0a | 185.6 ± 5.0a |
| 250 | 25.6 ± 3.5a | 187.0 ± 3.9a | |
aValues are Means ± SD (n = 3) followed by different letters with in a column are significantly different; Tukeys HSD, p ≤ 0.05; bMean number of spontaneous revertants as determined in assays without SH2 and standard mutagens; cMean number of revertants induced by reference mutagens, sodium azide and 4-Nitro-o-phenylenediamine (NPD); dMean number of revertants induced by DMSO in the absence of extract and mutagen; eMean number of revertants induced by SH2 at different concentrations.