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. 2016 Jun 29;7:921. doi: 10.3389/fpls.2016.00921

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Copyright © 2016 Belkheir, Gaid, Liu, Hänsch and Beerhues.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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BPS and CHS as well as flavonoids are located in the mesophyll. Immunofluorescence localization of CHS (A,B) and BPS (C,D) using a green fluorescent dye. (A,C) Cross-sections incubated with anti-His₆–CHS IgG and anti-His₆–BPS IgG, respectively. (B,D) Cross-sections incubated with the pre-immune IgGs. Localization of flavonoids (E,F) using histochemical detection. (E) Staining of flavonoids with diphenylboric acid 2-aminoethyl ester (DPBA). (F) Control section exhibiting only the red autofluorescence of chlorophyll. ue, upper epidermis; le, lower epidermis; m, mesophyll; vb, vascular bundle. Bar, 100 μm (A–D), 20 μm (E,F).