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Comparison of extraction methods
| Method | Peptides Identified | Proteins Identified | Average Peptides per Protein | Sequence Coverage (%) |
|---|---|---|---|---|
| NP40 | 14,000 ± 718 | 2270 ± 28 | 6.17 | 24.4 ± 0.4 |
| SDS/Freon | 3150 ± 801 | 1513 ± 155 | 2.08 | 6.6 ± 1.4 |
| 8 M Urea | 6660 ± 696 | 1820 ± 103 | 3.66 | 12.1 ± 1.2 |
Comparison of protein extraction methods. All methods using the FASP protocol for digestion of the proteins. The Q-Exactive HF mass spectrometer was used for analysis of these samples.
