Table 1.

Primer, probe, and positive template control sequences for the LSDV real-time PCR assay.

Primer/probe/PTC	Sequence
Forward primer	5′-TCC-GAG-CTC-TTT-CCT-TAC-TAT-3′
Reverse primer	5′-TAT-GGT-ACC-TAA-ATT-ATA-TAC-GTA-AAT-AAC-3′
Probea	5′6FAM-CAATGGGTAAAAGATTTCTA - MGBNFQ 3′
Positive Template Control	5′ ATG GCG ATG TCC ATT CCC TGA CCA ATG GGT AAA AGA TTT CTA TCG TAA CAG ATG AAA GAG CAA GCT ACT ATT CCT CAC GGA AAT GAA ATG CTT C 3′

^aFluorescent dye abbreviation: 6FAM = 6-Carboxyfluorescein; MGBNFQ = Minor groove binding, non-fluorescent quencher.