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. 2016 Jan 22;19(6):pyv139. doi: 10.1093/ijnp/pyv139

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© The Author 2016. Published by Oxford University Press on behalf of CINP.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Dorsal hippocampus (dHC) crude synaptosomal membrane phospholipase D (PLD)1/PLD2 isoforms show reduced phosphorylation states in the Day-1, while either decreased (PLD2) or unchanged (PLD1) phosphorylation and decreased PLD1/PLD2 expression in the Day-14 drug-free states in the paired group following conditioned hyperactivity (CH). 5-Hydroxytryptamine (5-HT) 2C receptor (5-HT_2CR) expression remains unchanged in Day-1 and decreases in the Day-14 drug-free state in the paired group following CH. Results represent the percent change in expression of the unpaired and paired compared to control group (±SEM). 5-HT_2CR expression in the paired group (A, hatched bars) is diminished by 50%, only in the Day-14 (dark background), not in the Day-1 (clear background) drug-free state compared with the unpaired group (slanted bars). Decreased phosphorylation states were observed in the paired group of animals (cross-hatch bars) compared with unpaired (slanted bars) at (B) pPLD1T147 (middle panel), pPLD1S561 (last panel) in PLD1 isoform, and (C) pPLD2Y169 (last panel) in the PLD2 isoform Day-1 (clear background) drug-free state. Phosphorylation states of the PLD1 isoform (B, middle and last panel) did not show any significant increase or decrease in paired group (cross hatched bars) in the Day-14 (dark background) drug-free state. However, the overall dHC crude synaptosomal expression for PLD1 (B, first panel) and PLD2 (C, first panel) levels were decreased by 50% in the paired (cross-hatched bars) compared with the unpaired (slanted bars) animals in the Day-14 (dark background) drug-free state. No changes in the paired (cross hatched bars) compared with unpaired (slanted bars) groups were observed in either PLD1 (B, first panel) or PLD2 (C, first panel) in the Day-1 (clear background) drug-free state. Diminished phosphorylation state of the PLD2 isoform Y169 (C, last panel) was observed in both the Day-1 (clear background) and Day-14 (dark background) drug-free states. ^# P<.05 vs paired; *P<.05 vs unpaired; n=8 animals/group.