Fig. 2.

MR diet decreased vBMD and BMC in mice. Bone densitometry was analyzed by μCT in mice fed either CF (white bars) or MR (black bars) diets. Cortical, trabecular and total vBMD and BMC in young males (A and E, respectively), young females (B and F, respectively), aged males (C and G, respectively), and aged females (D and H, respectively). Statistical analysis was conducted using Student's unpaired t-test (n = 7–8/group, *P < 0.05, **P < 0.01, ***P < 0.001). vBMD, volumetric bone mineral density, BMC, bone mineral content CF, control-fed; MR, methionine restriction.