TABLE 4.
FA analysis of RPMI B cells1
FAs | BSA | EPA | DHA |
14:0 | 0.9 ± 0.6 | 0.5 ± 0.5 | 0.6 ± 0.3 |
16:0 | 23.2 ± 1.4 | 16.0 ± 2.8 | 20.3 ± 1.3 |
16:1 | 4.0 ± 0.5 | 2.4 ± 0.8 | 3.1 ± 0.2 |
18:0 | 19.4 ± 0.4 | 15.9 ± 0.4** | 17.1 ± 0.4* |
cis 18:1 | 24.6 ± 3.4 | 17.5 ± 0.2 | 19.4 ± 0.2 |
18:2(n–6) | 1.8 ± 0.1 | 1.8 ± 0.1 | 1.8 ± 0.1 |
20:4(n–6) | 16.9 ± 3.9 | 7.2 ± 0.8* | 9.0 ± 0.3* |
20:5(n–3) | 0.4 ± 0.1 | 9.4 ± 0.3*** | 0.6 ± 0.1 |
22:5(n–3) | 4.7 ± 0.6 | 26.3 ± 2.3*** | 2.9 ± 0.1 |
22:6(n–3) | 4.0 ± 1.0 | 2.8 ± 0.4 | 25.0 ± 1.2*** |
∑ SFAs | 43.5 ± 1.7 | 32.4 ± 2.9* | 23.0 ± 1.2 |
∑ MUFAs | 28.8 ± 3.9 | 20.0 ± 0.6 | 22.6 ± 0.1 |
∑ (n–3) PUFAs | 9.0 ± 1.6 | 38.5 ± 2.7*** | 28.5 ± 1.1*** |
∑ (n–6) PUFAs | 18.7 ± 3.8 | 9.1 ± 0.8* | 10.8 ± 0.3 |
B cells were treated with BSA or 25 μmol EPA or DHA/L for 24 h. Data are means ± SEMs from 5 independent experiments. Values (arbitrary units) are percentage of total FAs. Asterisks indicate statistical significance relative to the BSA control: *P < 0.05, **P < 0.01, and ***P < 0.001.