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. 2016 Jun 15;30(12):1440–1453. doi: 10.1101/gad.277178.115

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© 2016 Zhou et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 2. — Ino80 silencing significantly inhibits melanoma growth in vitro. (A) Growth curves showing the impact of Ino80 silencing on melanoma cell lines. Melanoma cells were transduced with lentiviral-based nontargeting (NT) shRNA (shNT) or Ino80 shRNA (shIno80) and plated on day 1. Cell number (represented by fold change in absorbance) at the indicated time points was determined by MTT assay and normalized to that of day 1. Data were plotted as mean ± SEM from three independent experiments. (B) Representative images of the clonogenesis assay in melanoma cells transduced with NT shRNA or Ino80 shRNA virus. (C) Representative images showing anchorage-independent growth of melanoma cells transduced with NT shRNA or Ino80 shRNA virus. (D,E) Statistical analysis of the anchorage-independent growth assay showing changes in colony number (D) and colony size (E) of cells transduced with Ino80 shRNA relative to NT shRNA control.