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. Author manuscript; available in PMC: 2016 Jun 29.
Published in final edited form as: J Gene Ther. 2016 Feb 13;2(1):6. doi: 10.13188/2381-3326.1000006

Figure 1.

Figure 1

Construction of precursor miR-124 expression plasmids with optimized pre-miR-124 expression and mature miR-124 production. (A) Structures of pre-miR-124 expression plasmids. Seven plasmids were constructed with the same backbone consisting of an expression cassette with a CMV promoter and BGH polyA signaling sequences and a combination of three miR-124 precursor sequences. (B) The representative secondary structure of a tandem three pre-miR124 precursor miRNA segment with the lowest free-energy was predicted by RNAfold software (Vienna RNA WebServers). (C) Expression of mature miR-124 in in H1299, H322, and A549 cells transfected with different pre-miR124 constructs by real time qRT-PCR assay (mean ± SD) (n=3). Difference between two groups was analyzed by using Two-tailed t-test.