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. Author manuscript; available in PMC: 2016 Jun 29.

Published in final edited form as: Science. 2015 Oct 22;350(6262):826–830. doi: 10.1126/science.aab3145

Fig. 3 — In (A) to (C), mouse tissues were analyzed on day 3 after intraperitoneal (i.p.) infection with EMCV. (A) Quantitative PCR analyses of selected ISG mRNA expression in IECs and whole intestine. (B) Immunoblotting analyses of ISG protein abundance in whole intestine of co-housed mice. Right panel indicates relative ISG abundance normalized to a house keeping protein, Gapdh. (C) Quantitative PCR analyses of cellular EMCV loads and immune gene expression in MEFs after EMCV infection (MOI=0.1). (D) ELISA of IFN-β concentrations in the culture medium of MEFs after EMCV infection and quantification of infectious viral particles in the culture medium 16 hours after EMCV infection. (E) Left panel: the survival curves of WT and Nlrp6^−/− mice treated with 0.9% saline (mock) or 25 µg of recombinant mouse IFN-λ₂ 4h before oral infection with EMCV. Right panel: quantitative PCR analysis of EMCV loads in the whole blood cells 72 hours after infection. N=7–10/group, *P<0.05 (Log-rank test). In (A), and (E) the data are normalized with mouse beta actin and are presented as fold change over the mean of the results of WT (Mock-WT in E) mice. Each band/dot represents an animal. The horizontal lines in the figures indicate the median of the results. *P < 0.05, **P < 0.01 and ***P<0.001 (nonparametric Mann-Whitney analysis). In (C) and (D), bars: mean + S.E.M, n=3. *, P<0.05; **, P<0.01 (unpaired students’ t-test). ). The data are representative of /pooled from at least two independent experiments.

Fig. 3 — In (A) to (C), mouse tissues were analyzed on day 3 after intraperitoneal (i.p.) infection with EMCV. (A) Quantitative PCR analyses of selected ISG mRNA expression in IECs and whole intestine. (B) Immunoblotting analyses of ISG protein abundance in whole intestine of co-housed mice. Right panel indicates relative ISG abundance normalized to a house keeping protein, Gapdh. (C) Quantitative PCR analyses of cellular EMCV loads and immune gene expression in MEFs after EMCV infection (MOI=0.1). (D) ELISA of IFN-β concentrations in the culture medium of MEFs after EMCV infection and quantification of infectious viral particles in the culture medium 16 hours after EMCV infection. (E) Left panel: the survival curves of WT and Nlrp6^−/− mice treated with 0.9% saline (mock) or 25 µg of recombinant mouse IFN-λ₂ 4h before oral infection with EMCV. Right panel: quantitative PCR analysis of EMCV loads in the whole blood cells 72 hours after infection. N=7–10/group, *P<0.05 (Log-rank test). In (A), and (E) the data are normalized with mouse beta actin and are presented as fold change over the mean of the results of WT (Mock-WT in E) mice. Each band/dot represents an animal. The horizontal lines in the figures indicate the median of the results. *P < 0.05, **P < 0.01 and ***P<0.001 (nonparametric Mann-Whitney analysis). In (C) and (D), bars: mean + S.E.M, n=3. *, P<0.05; **, P<0.01 (unpaired students’ t-test). ). The data are representative of /pooled from at least two independent experiments.