TABLE 1 .
Strains used in this study
| Strain | Relevant genotype | Sourcea |
|---|---|---|
| B. subtilis | ||
| BD630 | his leu8 metB5 | Lab strain |
| BD3955 | his leu8 metB5 amyE::Pxyl-mreBCD Spc ΔmreB::Kan | This study |
| BD7587 | his leu8 metB5 mreB-K240Q | This study |
| BD7619 | his leu8 metB5 mreB-K240R | This study |
| E. coli | ||
| ED1881 | Stellar/pminimad2-mreBCD Amp | This study |
| ED1888 | Stellar/pminimad2-mreBCD-K240R Amp | This study |
| ED1895 | Stellar/pminimad2-mreBCD-K240Q Amp | This study |
a
The Pxyl-mreBCD and ΔmreB::Kan constructs were kindly provided by R. Daniels and J. Errington.