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. 2016 Jun 29;11(6):e0158141. doi: 10.1371/journal.pone.0158141

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© 2016 Spicer et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 7 — In both gnrh3^+/+ gnrh3:tdTomato (B) and gnrh3^-/- gnrh3:tdTomato (C) fish, Gnrh3-tdTomato-expressing soma located in the olfactory region and ventral telencephalon project fibers that extend posteriorly toward the hypothalamus and pituitary stalk (pituitaries not shown; A). Z-stack images were taken at 20x magnification on a Leica Microsystems DMi8 confocal microscope with a resolution of 1024 x 1024 and a z-step size of 0.10. All images were analyzed and assembled with Image J and Adobe Photoshop. Scale bars = 100 μm. OB = olfactory bulb. T = telencephalon. OT = optic tectum. ON = optic nerves. POA = pre-optic area. P = pituitary.