. 2016 Jun 29;11(6):e0158141. doi: 10.1371/journal.pone.0158141

Table 1. PCR primers for a two-step PCR process used to screen F3 fish fin-clip gDNA as either gnrh3^+/+, gnrh3^+/-, or gnrh3^-/- and to submit for sequencing.

Primer	PCR Step	Type	Sequence (5' → 3')	T_m (°C)	GC %	Amplicon size (bp)^*
Step1mutantFor	1	Mutant For	TTAGTTTAGATTTCAGCAGTTTTAGCATCATA	55.3	28.1	544
Step1WTFor	1	WT For	GAAGGTTGCTGGTCCAGTTGTTGCTG	62.2	53.8	582
Step2For	2	For	TTGAGATGGAAGACAATCCTTT	51.9	36.4	800
StepRev	1 and 2	Rev	TGCACATGTACTTGCTGAATTA	52.6	36.4

*when paired with StepRev primer.

Table 1. PCR primers for a two-step PCR process used to screen F3 fish fin-clip gDNA as either gnrh3+/+, gnrh3+/-, or gnrh3-/- and to submit for sequencing.