Table 2. QPCR primers used to quantify gene transcripts in gnrh3^+/+ and gnrh3^-/- fish.

Gene	Type	Sequence (5’ → 3’)	Tm (°C)	GC%	Amplicon Size (bp)
fshb	For	GCTGGACAATGGATCGAGTTTA	54.9	45.5	92
fshb	Rev	CTCGTAGCTCTTGTACATCAAGTT	54.5	41.7
lhb	For	GGCTGGAAATGGTGTCTTCT	55.1	50.0	107
lhb	Rev	CCACCGATACCGTCTCATTTAC	55.1	50.0
cga	For	TCCGGTCTATCAGTGCGT	55.6	55.6	148
cga	Rev	GGATATTCGTGGCAACCATTT	53.5	42.9
ef1a	For	AAGACAACCCCAAGGCTCTCA	58.6	52.4	255
ef1a	Rev	CCTTTGGAACGGTGTGATTGA	55.5	47.6