Figure 3.

CLSM images of P. aeruginosa N6P6 biofilm growth. (A) Untreated, without tannic acid (arrow indicating EPS portions); (B) Z stack image of untreated biofilm; (C) Biofilm treated with 0.3 mg/ml tannic acid; (D) Z stack image of tannic acid (0.3 mg/ml) treated biofilm. Red stained area was significantly reduced in tannic acid treated biofilm indicating no or very less EPS. The bacterial cells were not affected as their number did not reduce after tannic acid treatment (green stained).