. 2016 Jun 13;7(3):145–154. doi: 10.1080/21655979.2016.1191705

Table 2.

Recombinant bacterial lignin-degrading peroxidases. Examples of successfully produced recombinant bacterial lignin-degrading peroxidases and the substrates that have been used. The activity of the enzymes (if stated) is shown in parentheses. ABTS: 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonate), DCP: 2,4-dichlorophenol, DMP: 2,6-dimethoxyphenol, DOPA: L-3,4-dihydroxyphenylalanine, VA: veratryl alcohol.

enzyme	origin	host organism	localization	substrate (activity)	Ref
extracellular lignin peroxidase	Streptomyces viridosporus	Pichia pastoris	extracellular	DCP (∼1.1 (AU) min⁻¹) DOPA	⁴⁹
DyP-type (Tfu_3078)	Thermobifida fusca	Escherichia coli	periplasm	Reactive Blue 19 (K_m 29 µM, k_cat 10 s⁻¹)	⁵⁰
TfuDyP	T. fusca	E. coli	intracellular	ABTS (K_m 0.86 ± 0.07 mM, k_cat 28.1 ± 1 s⁻¹) DCP (K_m 5.51 ± 0.5 mM, k_cat 2.86 ± 0.1 s⁻¹) guaiacolphenol (K_m 0.126 ± 0.01 mM, k_cat 0.136 ± 0.03 s⁻¹) pyrogallol (K_m 11.29 ± 1 mM, k_cat 6.63 ± 0.25 s⁻¹) Reactive Blue 4 (K_m 0.179 ± 0.01 mM, k_cat 1.88 ± 0.07 s⁻¹) guaiacylglycerol-β-guaiacyl etherKraft lignin	⁵¹
DyP (DyP2)	Amycolatopsis sp. 75iv2	E. coli	extracellular	k_cat/ K_m (M⁻¹ s⁻¹)ABTS ((6.6 ± 0.9) × 10⁶)Reactive Blue 5 ((7.1 ± 0.9) × 10⁵)Reactive Black 5 ((1.6 ± 0.1) × 10⁵)Mn²⁺ ((1.2 ± 0.2) × 10⁵)guaiacylglycerol-β-guaiacol ether,veratrylglycerol-β-guaiacol ether	⁵²
DyP-type (dyp Pa)	Pseudomonas aeruginosa	E. coli	intracellular	Reactive Blue 5 (4395 µM mg⁻¹ min⁻¹) Reactive Blue 19 (107 µM mg⁻¹ min⁻¹) Reactive Black 5 (704 µM mg⁻¹ min⁻¹) DMP (786 µM mg⁻¹ min⁻¹)	⁵³
DyP	Anabaena sp strain PCC7120	E. coli	intracellular	Reactive Blue 5 (K_m 3.6 M, k_cat/K_m 1.2 × 10⁷ M⁻¹ s⁻¹)	⁵⁴
PpDyPBsDyP	Pseudomonas putidaBacillus subtilis	E. coli	intracellular	(PpDyP // BsDyP) ABTS (40 ± 0.9 U mg⁻¹ // 15 ± 1 U mg⁻¹) Reactive Blue 5 (15 ± 0.2 U mg⁻¹ // 11 ± 0.6 U mg⁻¹) guaiacol (0.2 ± 0.004 U mg⁻¹ // 0.2 ± 0.006 U mg⁻¹) syringaldehyde (0.11 ± 0.005 U mg⁻¹ // 0.004 ± 0.0002 U mg⁻¹) acetosyringone (0.16 ± 0.008 U mg⁻¹ // 0.012 U mg⁻¹)	⁵⁵
BsDyP	B. subtilis	E. coli	intracellular	ABTS (66.80 U mg⁻¹) VA (0.13 U mg⁻¹) Reactive Blue 19 (11.55 U mg⁻¹) Reactive Black 5 (17.65 U mg⁻¹) veratrylglycerol-β-guaiacolether (0.086 U mg⁻¹)	⁵⁶
SviDyP	Saccharomonospora viridis	E. coli	intracellular	Reactive Blue 19 (1.29 U mg⁻¹) Reactive Green 19 (1.32 U mg⁻¹) Reactive Yellow 2 (4.86 U mg⁻¹) Reactive Black 5 (0.96 U mg⁻¹) Reactive Red 120 (0.69 U mg⁻¹) Brilliant Green (12.24 U mg⁻¹) Malachite Green (8.4 U mg⁻¹) Crystal Violet (4.11 U mg⁻¹) Azure B (1.62 U mg⁻¹) DMP (0.06 U mg⁻¹) VA (0.03 U mg⁻¹) eucalyptus kraft pulp (Kappa decrease 21.8 %)	⁵⁷
DyP1B	Pseudomonas fluorescens	E. coli	intracellular	ABTS (K_m 1.13 ± 0.1 mM, k_cat 13.5 ± 0.4 s⁻¹) DCP (K_m 1.25 ± 0.1 mM, k_cat 0.66 ± 0.02 s⁻¹) guaiacol (K_m 0.056 ± 0.006 mM, k_cat 0.058 ± 0.001 s⁻¹) pyrogallol (K_m 4.0 ± 0.6 mM, k_cat 2.5 ± 0.1 s⁻¹) Reactive Blue 4 (K_m 0.12 ± 0.01 mM, k_cat 1.04 ± 0.03 s⁻¹) Kraft lignin (K_m 0.006 ± 0.001 mM, k_cat 0.9 ± 0.1 s⁻¹)	⁵⁸
DypB	Rhodococcus jostii	E. coli	intracellular	ABTS (0.0549 ΔAbs min⁻¹) β-aryl ether lignin model Kraft lignin (0.24 ΔAbs 10 min⁻¹) nitrated lignin (0.0081 ΔAbs 20 min⁻¹) wheat straw lignocellulose	⁵⁹
DyPA	R. jostii	E. coli	intracellular	ABTS (K_m 8.2 mM, k_cat 16.83 s⁻¹)	⁶⁰
peroxidase	R. jostii	R. jostii	extracellular	wheat straw lignocellulose (96 mg L⁻¹ vanillin53 mg L⁻¹ p-hydroxybenzaldehyde 3-120 mg L⁻¹ vanillic acid 23-86 mg L⁻¹ ferulic acid)	⁶¹