FIGURE 6:

Triacylglycerol lipase activity and lysophosphatidic acid acyltransferase activity in microsomes from strains compromised in nonpolar lipid metabolism. For these experiments, microsomes from dga1∆lro1∆are1∆are2∆ (QM), dga1∆lro1∆are1∆are2∆tgl4∆ (QM tgl4∆), and dga1∆lro1∆are1∆are2∆tgl5∆ (QM tgl5∆) were isolated (for details, see Materials and Methods). TG lipase activity (A) and lysophosphatidic acid acyltransferase activity (B) in 30,000 × g ER fractions from QM (black bar), QM tgl4∆ (dark gray bar), and QM tgl5∆ (light gray bar) strains were analyzed. Assays were performed in triplicate from at least two independent biological samples. Data are mean values with the respective deviation. Significance was calculated by Student’s t-test (two tailed, unpaired). ***p < 0.001, defined to be significant.