FIGURE 7:

The delivery of Htt103QP into vacuoles is compromised in dsk2Δ mutants. pep4Δ VPH1-mApple and dsk2Δ pep4Δ VPH1-mApple cells with Htt103QP plasmid were grown in galactose medium for 12 h, and then glucose (2%) and HU (200 mM) were added into the medium to shut off Htt103QP expression and block cell cycle progression. The cells were collected at 2 and 4 h after glucose addition to examine the GFP and mApple signals. (A) Representative cell images for the localization of Htt103QP-GFP and Vph1-mApple (2 h), as well as the percentage of the colocalization (n > 100). (B) Statistical dot plot of the intensity of GFP and mApple signals within the vacuole of WT and dsk2Δ mutant cells (n = 20) and medians (black bars). The p values are from the statistical comparison between WT and dsk2Δ cells.