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. 2016 Jul 1;27(13):2133–2144. doi: 10.1091/mbc.E15-07-0490

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© 2016 Loucks, Bialas, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — C. elegans pcrg-1 animals show a significant decrease in lifespan compared with wild type. (A) Both alleles of pcrg-1 (gk480, tm2597) show a significant decrease in lifespan compared with the wild-type N2 strain. (B) Expression of PCRG-1::GFP (construct A with 991– base pair promoter) rescues the lifespan defect shown in the gk480 allele. (C) Expression of PCRG-1::GFP (construct B with 3182–base pair promoter) rescues the lifespan defect shown in the tm2597 allele. (D) Expression of PCRG-1::GFP (construct B) in the wild-type (N2) background does not affect the lifespan of the worm. For number of animals and p values comparing differences between strains in lifespan experiments, see Supplemental Table S1.