Author response image 1. ATP:ADP ratio in yeast cell extracts.
Yeast cells (diploid strain X2180) were grown at 30°C to 1.4 * 108 cells ml-1 in YPD (A) or 0.7 * 108 cells ml-1 in YPGal medium (B). Cells were quickly harvested through centrifugation (5000 g, 1’), washed twice in distilled water and resuspended in 0.2 M perchloric acid (400 μl per 100 mg of wet weight). Cell lysis was performed by two series of freezing/thawing and the extracts were neutralized by adding potassium phosphate (3M) to the final pH 7. Extracts were centrifuged twice (10 000 g, 10’) and supernatant was analyzed using reversed-phase high performance liquid chromatography (RP-HPLC) according to (Smolenski et al, 1990) on GBC 1150 HPLC Pump, Spectra System AS3000 autosampler, Thermo Finnigan Spectra System UV6000LP. The separation was performed on 50 x 4,6 mm HyperClone 3u BDS C18, 130 A column (Phenomenex).