Figure 2. Munc13-1 C₁C₂BMUN clusters PS-containing liposomes.

(A–E) The particle size in samples containing phospholipid vesicles alone (gray bars) or after incubation with Munc13-1 C₁C₂BMUN for 5 min (red bars) in the absence of Ca²⁺ was measured by DLS. The liposomes had a standard lipid composition including no PS (A), PS (B), PS and synaptobrevin (C), PS+DAG+PIP₂ (D) or PS and syntaxin-1 (E). (F) Intensity autocorrelation curves corresponding to the experiments shown in (A–E) after incubation with Munc13-1 C₁C₂BMUN for 5 min.

DOI: http://dx.doi.org/10.7554/eLife.13696.005

Figure 2—figure supplement 1. Ca²⁺ does not stimulate liposome clustering by C₁C₂BMUN strongly.

The diagram shows intensity autocorrelation curves measured by DLS at 25°C for PS-containing vesicles alone or after 5 min incubation with C₁C₂BMUN in the presence of 100 μM EGTA or 500 μM Ca²⁺.

DOI: http://dx.doi.org/10.7554/eLife.13696.006