| Lane M | Lane 1 | Lane 2 | Lane 3 | Lane 4 | |
|---|---|---|---|---|---|
| Loading buffer (6×, μl) | — | 2 | 2 | 2 | 2 |
| Primer stock solution (μl) | — | — | 2 | 1 | — |
| Template stock solution (μl) | — | 2 | — | 1 | — |
| NF stock solution (μl) | — | — | — | — | 2 |
| Ladder (20 bp, μl) | 3 | — | — | — | — |
| DPBS (μl) | — | 6 | 6 | 6 | 6 |
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