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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Nat Protoc. 2015 Sep 10;10(10):1508–1524. doi: 10.1038/nprot.2015.078
Samples incubated with nanoflowers
1 2 3 4 5 6 7 8
Nanoflower (U) 0.01 0.02 0.04 0.08 0.16 0.32 0.64 1.28
Free Dox — — — — — — — —
Nanoflower-Dox complex — — — — — — — —
Samples incubated with free Dox
1′ 2′ 3′ 4′ 5′ 6′ 7′ 8′
Nanoflower — — — — — — — —
Free Dox (μM) 0.1 0.2 0.4 0.8 1.6 3.2 6.4 12.8
Nanoflower-Dox complex — — — — — — — —
Samples incubated with nanoflower-Dox complex
1″ 2″ 3″ 4″ 5″ 6″ 7″ 8″
Nanoflower — — — — — — — —
Free Dox — — — — — — — —
Nanoflower-Dox complex (U) 0.01 0.02 0.04 0.08 0.16 0.32 0.64 1.28
See Step 70 for the definition of U.