Table 1. MAP salt-cue conditioning procedure in N2 adult C. elegans.

Worms were washed off plates with RO water and transferred to 15 ml centrifuge tubes. The supernatant was removed to remove any remaining bacteria. 200 μl of worms were added to each 0.45um filter. Worms were exposed to water or conditioning solutions in a Swinnex® filter holder connected to a peristaltic pump using a flow rate of 1.0 ml/min. First, worms were washed with water for 5 min followed by a 2 min wash using a conditioning solution (CS+). Then worms were washed with water again for 5 min followed by a 2 min wash using another conditioning solution (CS-). This process was repeated until worms were exposed to a total of 5 water washes and 4 conditioning solution washes (2 CS+ and 2 CS-). Following this conditioning procedure, worms were washed off filters into 15 ml tubes and the supernatant was removed. Then 4ul of worms were pipetted into the center of each well of a 6-well testing plate and pictures of each well were taken 60 min after placing worms on test plates.

Initial Wash	Conditioning								Testing
5 min	2 min	5 min	2 min	5 min	2 min	5 min	2 min	5 min	60 min
Water Wash	Drug + CS+	Water Wash	CS-	Water Wash	Drug + CS+	Water Wash	CS-	Water Wash	Salt Preference Testing