Table 2. Food conditioning procedure in N2 adult C. elegans.

Worms were washed off plates with water and transferred to 15 ml centrifuge tubes. The supernatant was removed to remove any remaining bacteria. Worms were placed on CS- conditioning plates for 3 hrs and washed off plates with water. Worms were then placed on CS+ plus food or CS+ no food plates for 1hr. Following this conditioning procedure, worms were washed off plates and 4ul of worms were pipetted into the center of each well of a 6-well testing plate and pictures of each well were taken 30 min after placing worms on test plates.

Wash off Maintenance Plates	Conditioning				Testing
	3 hr		1 hr		30 min
Water Wash	CS- no food	Water Wash	CS+ plus Food or CS+ no food	Water Wash	Salt Preference Testing