Figure 2.

Neutrophils are increased in the lungs of mice lacking myeloid NF-κB signaling. A) Number of total BAL cells in WT and IKKβ^Δmye mice at baseline (C) and at 6 weeks after urethane injection (U) (n=7-9 mice per group; *p < 0.05 compared with urethane-treated WT mice). B) Representative FACS plots and (C-D) Percentages of viable CD45⁺/CD11b⁺/Gr1^hi neutrophils (Gr1^hi), CD45⁺/CD11b⁺/Gr1^low monocytes (Gr1^low), and CD45⁺/CD11b⁺/Gr1^neg macrophages (Gr1^neg) in the lungs of WT and IKKβ^Δmye mice at (C) baseline and (D) 6 weeks after urethane injection (n=4-11 mice per group; *p < 0.05 compared with WT). E) Representative FACS plots and (F) total viable CD45⁺/CD11b⁺/Ly6G⁺ neutrophils in the lungs of WT→Kras^G12D and IKKβ^Δmye→Kras^G12D mice 8 weeks after adeno-Cre (n=4 mice per group; *p < 0.05 compared with WT→Kras^G12D). Ly6G identifies the granulocytic subgroup of the Gr1 marker. See also Figure S2.