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. Author manuscript; available in PMC: 2016 Jul 7.
Published in final edited form as: Cell Rep. 2016 Jun 16;16(1):120–132. doi: 10.1016/j.celrep.2016.05.085

Figure 3.

Figure 3

Neutrophils promote lung tumorigenesis in the absence of myeloid NF-κB signaling. All mice were treated with isotype control IgG or anti-Ly6G depletion antibodies (100 g by IP injection) for the first 6 weeks following urethane injection. A) Representative FACS plots and (B) total viable CD45+/CD11b+/Ly6C+/Ly6G+ lung neutrophils demonstrating depletion efficiency in IKKβΔmye mice harvested 3 days after the last dose of antibody (n=4 mice per group). C) Number of AAH lesions per lung section from IgG- and anti-Ly6G-treated WT and IKKβΔmye mice at 6 weeks after urethane injection (n=6-9 mice per group). D) Lethally-irradiated WT mice received bone marrow from WT or IKKβΔmye mice. Lung tumors at 16 weeks after urethane injection in bone marrow chimera mice treated with IgG or anti-Ly6G antibodies for the first 6 weeks of tumorigenesis (n=6-8 mice per group). *p < 0.05.