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A. SthAM is enzymatically prepared from ClDthA and l-methionine using SalL. B. Methylation of linearized pUC19 DNA with M.TaqI and SthAM is followed by a strand cleavage step with TaqI (a restriction enzyme). C. Reactions are then analyzed by agarose gel electrophoresis. Methylation (protection) prevents any digestion and shows full length DNA. Unmethylated DNA shows full digestion after the exposure to a restriction enzyme.
