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Methylation of linearized pUC19 DNA by M.TaqI MTase. A DNA marker (100–3000 bp) was used as a reference (lane L). Lane 1 and 2 show the result after the reaction using SthAM and SAM respectively. Negative controls in the absence of M.TaqI for the reaction with SthAM (lane 3), SAM (lane 4) or no cofactor (lane 5) are shown. Lane 6 shows a control reaction in absence of any cofactor or enzyme.
