Fig. 1. SVCT-2 expression in human T cells. (A) Total RNA was extracted from human T cells and cDNA was synthesized. The expression of SVCT-1 and -2 was assessed using reverse transcription polymerase chain reaction. (B) T cells were lysed and subjected to western blotting for the expression of SVCT-2. HepG2 (hepatoma cell line) cells were used as positive control of SVCT-1 and -2. (C) Human T cells were collected 24 hours or 48 hours after activation with phorbol-12-myristate-13-acetate/ionomycin, attached on a slide, and briefly fixed with formalin. The expression of SVCT-2 was assessed by immunofluorescence staining. The staining was observed under a confocal microscope.
