Skip to main content
. 2016 Jun 29;5(1):924. doi: 10.1186/s40064-016-2614-6

Table 1.

Primer pair sequences used for real-time PCR amplifications

RT-PCR primer 5′–3′ Annealing temperature (°C)
HSL F ACAGCAGTCACGGTTTGGT 60
HSL R AGAGGAAGGCGTAGAGGGA
PEPCK F GAACGGCTTCTTCGGTGT 60
PEPCK R AAGCGGGAGTTGGGGTG
β-actin F GGAAATCGTGCGTGACATTAAG 60
β-actin R CCTCTGGACAACGGAACCTCT

HSL hormone-sensitive lipase; PEPCK phosphoenolpyruvate carboxykinase