Table 1.
RT-PCR primer | 5′–3′ | Annealing temperature (°C) |
---|---|---|
HSL F | ACAGCAGTCACGGTTTGGT | 60 |
HSL R | AGAGGAAGGCGTAGAGGGA | |
PEPCK F | GAACGGCTTCTTCGGTGT | 60 |
PEPCK R | AAGCGGGAGTTGGGGTG | |
β-actin F | GGAAATCGTGCGTGACATTAAG | 60 |
β-actin R | CCTCTGGACAACGGAACCTCT |
HSL hormone-sensitive lipase; PEPCK phosphoenolpyruvate carboxykinase