Fig. 3.

VCP promotes proliferation of CRC in vivo. a Representative images of xenograft tumors were shown for the indicated CRC cells. The histogram showed the size of tumors for the indicated CRC cells. Values are expressed as the mean ± SD of experiments performed in triplicate. b Tumors from the different groups were immunostained for cleaved-caspase-3, CD31 and Ki-67. Images are representative of three independent experiments. c Quantification of immunostaining in (b). CD31-stained microvessels were counted to record microvessel density, apoptotic cells were counted to give the apoptosis index and cells expressing Ki-67 were counted to calculate the Ki-67 positive cells. d Western blot was performed to detect the protein expression levels of the indicated molecules from tumor samples. Actin was used as the internal control. Western blot was repeated three times