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. 2016 Jun 30;7:952. doi: 10.3389/fpls.2016.00952

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Copyright © 2016 Kondo, Hori, Sasaki-Sekimoto, Kobayashi, Kato, Yuno-Ohta, Nobusawa, Ohtaka, Shimojima and Ohta.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Klebsormidium flaccidum lipid recovery yields according to the different extraction time points. The K. flaccidum lipids were extracted by chloroform. Solvent was collected at predefined times and fresh solvent was added immediately. Closed circles with solid lines indicate TAG, whereas open circles with dotted lines indicate MGDG. The inset shows lipid recovery per dry weight, and the solid and dotted straight lines represent TAG and MGDG, respectively, which were both derived using the Bligh and Dyer method (Bligh and Dyer, 1959). Values represent means ± SD (n = 3).