FIGURE 5.

Lipid separation extracted from K. flaccidum and A. thaliana using the chloroform extraction method. (A) Comparison of lipid separation between K. flaccidum and A. thaliana. The lipids of K. flaccidum were extracted once with chloroform for 30 s, whereas those of A. thaliana were extracted twice with chloroform and the resulting lipids were pooled. Lipids were separated on a TLC plate using a solvent system containing hexane/diethyl ether/acetic acid (90:7.5:1, v/v/v), and visualized with 0.01% (w/v) primuline in 80% (v/v) acetone under ultraviolet light. (B) Lipid separation of K. flaccidum. Lipids extracted with chloroform as in (A) were separated on a TLC plate using the following solvent system: hexane to a height of 20 cm and, toluene to a height of 20 cm, followed by hexane/diethyl ether/acetic acid (70:30:1, v/v/v) to a height of 10 cm.