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. 2016 Jun 30;7:952. doi: 10.3389/fpls.2016.00952

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Copyright © 2016 Kondo, Hori, Sasaki-Sekimoto, Kobayashi, Kato, Yuno-Ohta, Nobusawa, Ohtaka, Shimojima and Ohta.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Wax extracted from A. flaccidum using the silica gel extraction method. (A) Left, top to bottom: 28 days old and 42 days old K. flaccidum cultures, and 14 days old C. reinhardtii. Right: lipid recovery from silica gel plate extractions for K. flaccidum and C. reinhardtii. (B) Separation of lipid from K. flaccidum and C. reinhardtii. Lipids derived by silica gel plate extraction were separated on a TLC using the following solvent system: hexane to a height of 20 cm and, toluene to a height of 20 cm, followed by hexane/diethyl ether/acetic acid (70:30:1, v/v/v) to a height of 10 cm.