Table 1.
PCR protocols used in this study.
| Gene | Primers (5′–3′) | Size (bp) | Annealing temperature (°C) | Reference |
|---|---|---|---|---|
| ddlE. faecium | F-TTGAGGCAGACCAGATTGACG R-TATGACAGCGACTCCGATTCC |
658 | 54 | Macovei and Zurek19 |
| ddlE. faecalis | F-TCAAGTACAGTTAGTCTTTATTAG R-ACGATTCAAAGCTAACTGAATCAGT |
941 | 54 | Macovei et al.19 |
| 16S rDNA | F-GGATTAGATACCCTGGTAGTCC R-TCGTTGCGGGACTTAACCCAAC |
320 | 54 | Macovei et al.19 |
| aac(6′)-Ie-aph(2″)-Ia | F-CAGGAATTTATCGAAAATGGTAGAAAAG R-CACAATCGACTAAAGAGTACCAATC |
369 | 56 | Emaneini et al.23 |
| aph(2″)-Ib | F-CTTGGACGCTGAGATATATGAGCAC R-GTTTGTAGCAATTCAGAAACACCCTT |
867 | 56 | Emaneini et al.23 |
| aph(2″)-Ic | F-CCACAATGATAATGACTCAGTTCCC R-CCACAGCTTCCGATAGCAAGAG |
444 | 56 | Emaneini et al.23 |
| aph(2″)-Id | F-GTGGTTTTTACAGGAATGCCATC R-CCCTCTTCATACCAATCCATATAACC |
641 | 56 | Emaneini et al.23 |
| aph(3′)-IIIa | F-GGCTAAAATGAGAATATCACCGG R-CTTTAAAAAATCATACAGCTCGCG |
523 | 56 | Emaneini et al.23 |
| ant(6′)-Ia | F-ACTGGCTTAATCAATTTGGG R-GCCTTTCCGCCACCTCACCG |
577 | 56 | Sepúlveda et al.22 |
| cylA | F-TGGATGATAGTGATAGGAAGT R-TCTACAGTAAATCTTTCGTCA |
517 | 54 | Reviriego et al.21 |
| gelE | F-AGTTCATGTCTATTTTCTTCAC R-CTTCATTATTTACACGTTTG |
402 | 56 | Duprè et al.20 |
| esp | F-TTACCAAGATGGTTCTGTAGGCAC R-CCAAGTATACTTAGCATCTTTTGG |
936 | 56 | Duprè et al.20 |
| ace | F-AAAGTAGAATTAGATCCACAC R-TCTATCACATTCGGTTGCG |
320 | 56 | Duprè et al.20 |
| agg | F-CCAGTAATCAGTCCAGAAACAACC R-TAGCTTTTTTCATTCTTGTGTTTGTT |
406 | 53 | Duprè et al.20 |
| efaA | F-CGTGAGAAAGAAATGGAGGA R-CTACTAACACGTCACGAATG |
499 | 56 | Duprè et al.20 |