Supplemental Fig. S1.

Effect of different oils on the Acinetobacter sp. AU07 lipase activity. The enzyme production was carried out with different oils as inducers, viz. castor oil, palm oil, coconut oil, sesame oil, and olive oil, at (1%, v/v) with a 1% inoculum size at 30 °C for 20 h in a rotary shaker (150 rpm). The media containing oils were emulsified with 0.25% gum acacia. The initial pH of the media was adjusted to 7.0. To the individual effects of pH, temperature and inducers were monitored and optimized. The cell-free supernatant was recovered by centrifugation at 12,500 × g for 10 min at 4 °C and used to determine the extracellular lipase activity.