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RP-HPLC analysis of the purified Acinetobacter sp. AU07 lipase. The column was eluted with the following buffers: 0.1% (v/v) trifluroacetic acid (TFA) in water and 0.1% (v/v) TFA in acetonitrile. The bound proteins were eluted with an increasing gradient of 2–100% acetonitrile for 40 min at a flow rate of 1 mL/min.
