Response surface plots of the impact of various factors on optimal production of the AU07 lipase. Each figure depicts the impact of specific factors on lipase production. The variables investigated were temperature, pH, agitation, inducer concentration, and inoculum volume. (A) Temperature and pH, (B) temperature and agitation, (C) temperature and inducer, (D) temperature and inoculum volume, (E) pH and agitation, (F) pH and inducer, (G) pH and inoculum volume, (H) agitation and inducer, (I) agitation and inoculum volume, and (J) inducer and inoculum volume.