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. 2016 Apr 26;47(3):647–657. doi: 10.1016/j.bjm.2015.04.002

Fig. 2.

Fig. 2

Growth curve and lipase production from Acinetobacter sp. AU07 in shake flask and bioreactor. (A) Growth curve and lipase production from Acinetobacter sp. AU07 in a shake flask (30 °C, pH 7.0, 0.5%, v/v inoculum size, 2%, v/v inducer concentration, 150 rpm). The lipase activity was measured using 1 mM 4-NP as the substrate. The reaction was incubated at 30 °C for 10 min, and the absorbance was measured at 410 nm. The OD was converted to lipase activity (U/mL) and the graph was plotted. Each data point is the mean of three replicates. (B) Growth curve and production of lipase from Acinetobacter sp. AU07 in a bioreactor (3 L) (30 °C, pH 7.0, 0.5%, v/v inoculum size, 2%, v/v inducer concentration, 150 rpm, 1.5 vvm aeration). The lipase activity was measured using 4-NP (1 mM) as the substrate. The reaction was incubated at 30 °C for 10 min and OD was measured at 410 nm. The OD was converted to lipase activity (U/mL) and the graph was plotted. Each data point is the mean of three replicates.