Table 1.
Experimental designs used in RSM studies with two independent variables showing the observed and the predicted values of lipase production.
| Std | Run | Factor 1 | Factor 2 | Factor 3 | Factor 4 | Factor 5 | Observed activity (U/mL) | Predicted Lipase activity (U/mL) |
|---|---|---|---|---|---|---|---|---|
| 1 | 26 | 40.00 | 8.00 | 100.00 | 3.00 | 0.25 | 6.20 | 6.36 |
| 2 | 3 | 40.00 | 6.00 | 200.00 | 3.00 | 0.25 | 6.60 | 6.76 |
| 3 | 8 | 20.00 | 8.00 | 200.00 | 1.00 | 0.75 | 4.40 | 4.56 |
| 4 | 11 | 40.00 | 8.00 | 200.00 | 1.00 | 0.25 | 6.00 | 6.16 |
| 5 | 1 | 40.00 | 8.00 | 100.00 | 1.00 | 0.75 | 8.65 | 8.81 |
| 6 | 13 | 40.00 | 6.00 | 100.00 | 3.00 | 0.75 | 5.32 | 5.48 |
| 7 | 5 | 20.00 | 6.00 | 200.00 | 3.00 | 0.75 | 4.13 | 4.29 |
| 8 | 12 | 20.00 | 8.00 | 100.00 | 3.00 | 0.75 | 5.40 | 5.56 |
| 9 | 22 | 40.00 | 6.00 | 200.00 | 1.00 | 0.75 | 4.60 | 4.76 |
| 10 | 4 | 20.00 | 8.00 | 200.00 | 3.00 | 0.25 | 7.73 | 7.79 |
| 11 | 25 | 20.00 | 6.00 | 100.00 | 1.00 | 0.25 | 2.42 | 2.74 |
| 12 | 10 | 20.00 | 7.00 | 150.00 | 2.00 | 0.50 | 4.32 | 4.02 |
| 13 | 14 | 40.00 | 7.00 | 150.00 | 2.00 | 0.50 | 8.40 | 8.10 |
| 14 | 24 | 30.00 | 6.00 | 150.00 | 2.00 | 0.50 | 6.12 | 5.82 |
| 15 | 15 | 30.00 | 8.00 | 150.00 | 2.00 | 0.50 | 9.40 | 9.10 |
| 16 | 9 | 30.00 | 7.00 | 100.00 | 2.00 | 0.50 | 11.50 | 11.20 |
| 17 | 19 | 30.00 | 7.00 | 200.00 | 2.00 | 0.50 | 12.32 | 12.02 |
| 18 | 20 | 30.00 | 7.00 | 150.00 | 1.00 | 0.50 | 8.42 | 8.12 |
| 19 | 21 | 30.00 | 7.00 | 150.00 | 3.00 | 0.50 | 11.00 | 10.70 |
| 20 | 18 | 30.00 | 7.00 | 150.00 | 2.00 | 0.25 | 4.20 | 4.00 |
| 21 | 7 | 30.00 | 7.00 | 150.00 | 2.00 | 0.75 | 13.20 | 12.90 |
| 22 | 2 | 30.00 | 7.00 | 150.00 | 2.00 | 0.50 | 14.50 | 14.65 |
| 23 | 23 | 30.00 | 7.00 | 150.00 | 2.00 | 0.50 | 14.50 | 14.65 |
| 24 | 6 | 30.00 | 7.00 | 150.00 | 2.00 | 0.50 | 14.50 | 14.65 |
| 25 | 16 | 30.00 | 7.00 | 150.00 | 2.00 | 0.50 | 14.50 | 14.65 |
| 26 | 17 | 30.00 | 7.00 | 150.00 | 2.00 | 0.50 | 14.50 | 14.65 |
The table includes the factors influencing lipase production. Factor 1: temperature (°C), Factor 2: pH, Factor 3: agitation (rpm), Factor 4: inducer concentration (%).