Table 5.

Effect of organic solvents on the Acinetobacter sp. AU07 lipase.

Solvents	Residual activity (%)
	Incubation (2 h)	Incubation (4 h)
Control	100.00	100.00
Methanol	60.40 ± 1.10	44.15 ± 1.85
Ethanol	56.25 ± 3.95	35.60 ± 4.40
1-Propanol	9.80 ± 2.50	8.60 ± 3.40
1-Butanol	25.20 ± 1.00	14.50 ± 0.50
Acetonitrile	44.85 ± 0.15	39.0 ± 1.00
1-Hexane	137.15 ± 3.05	109.15 ± 2.95
Toluene	356.30 ± 5.90	281.10 ± 5.85
Dichloromethane	113.50 ± 1.50	46.25 ± 2.75
1-Heptane	106.10 ± 3.90	77.60 ± 0.40
Dimethylsulfoxide	190.70 ± 4.30	181.00 ± 1.00

The enzyme was pre-incubated for 2 and 5 h with different organic solvents (30%, v/v) while shaking (150 rpm) at 30 °C and assayed under standard assay conditions. The stability of the lipase was calculated by comparing the residual activity with that of the control, which was incubated without the organic solvent and whose enzyme activity was taken as 100%. The standard deviation for all the values is <±5.0%.