. 2016 Apr 26;47(3):647–657. doi: 10.1016/j.bjm.2015.04.002

Table 6.

Effect of metal ions on the activity of the Acinetobacter sp. AU07 lipase.

Metal ions	Residual activity (%)
	2 mM	5 mM	10 mM
Zn²⁺	5.58 ± 0.08	4.92 ± 0.12	3.26 ± 0.04
Mg²⁺	51.00 ± 1.00	34.20 ± 1.20	16.45 ± 1.45
Ca²⁺	19.90 ± 0.90	10.05 ± 0.75	3.81 ± 0.21
Ag²⁺	62.00 ± 0.40	47.85 ± 0.45	18.25 ± 0.45
Fe³⁺	56.72 ± 0.42	32.70 ± 0.30	21.20 ± 0.60
Mn²⁺	36.10 ± 0.80	11.97 ± 0.47	0.82 ± 0.11

The pure enzyme was incubated with metal salts of different concentrations, and the residual enzyme activity was determined under standard assay conditions. The enzyme activity without metal salts was taken as 100%. Each value presented here is an average of three replicates.